Preparation and characterization of three monoclonal antibodies against HIV-1 p24 capsid protein.

نویسندگان

  • Guang Jie Liu
  • Jian Ping Wang
  • Jian Chun Xiao
  • Zhi Wei Zhao
  • Yong Tang Zheng
چکیده

HIV-1 p24 detection provides a means to aid the early diagnosis of HIV-1 infection, track the progression of disease and assess the efficacy of antiretroviral therapy. In the present study, three monoclonal antibodies (mAbs) p3JB9, p5F1 and p6F4 against HIV-1 p24 were generated. All mAbs could detect p24 of HIV-1IIIB, HIV-1Ada-M, HIV-174V mAbs p5F1 and p6F4 could detect HIV-1KM018, while p3JB9 could not. Three mAbs did not react with HIV-2ROD, HIV-2CBL-20 and SIVagmTYO-1. The recognized epitope of p5F1 was located on the Gag amino acid region DCKTILKALGPAATLEEMMTAC. The p5F1 was used to establish a modified sandwich ELISA with rabbit anti-p24 serum and showed good specificity and high sensitivity, which has been used to measure HIV-1 p24 antigen levels in research.

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عنوان ژورنال:
  • Cellular & molecular immunology

دوره 4 3  شماره 

صفحات  -

تاریخ انتشار 2007